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|
| TR |
TITLE |
AUTHORS |
KEYWORDS |
MATERIALS & METHODS
|
MICROSCOPIC
TECHNIQUES |
SPECIES |
MORPHOLOGY |
CELL
LINE |
| 618 |
Regulation
Of SP2 DNA-Binding Activity And trans-Activation |
K.
Scott Moorefield |
transcription,
Sp-family of DNA-binding proteins, trans-activation |
For confocal microscopy, 2-4
x 104 COS-1 cells were plated in 35 mm glass-bottom dishes (MatTek Corp., Ashland, MA) and cultured
overnight at 37 °C in DMEM supplemented with heat-inactivated FBS, and 50 µg/ml Pipracil at
37 °C under 5% CO2.
|
confocal
microscopy |
|
|
COS-1 |
| |
Regulated transcription requires the collaboration
of a variety of transcription factors, including sequence-specific DNA-binding proteins. The
Sp-family of DNA-binding proteins governs the expression of a wide variety of mammalian genes,
including housekeeping, cell cycle-regulated, and developmentally regulated genes. A wealth
of information has been obtained about the functional and biochemical properties of two Sp-family
members, Sp1 and Sp3, |
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|
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