Glass Bottom Culture Dishes



35mm and 50 mm dishes





Glass Bottom
Multiwell Plates



6, 12, 24 & 96-well plates

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Frequently Asked Questions
Multi-Well Glass Bottom Culture Plates

 

Click on a subject heading below to view questions and answers relating to your selection. Links throughout the answers will guide you to further information on our website or from other sources. Should you have any further questions, please contact MatTek Corporation at DishInfo

Q 1:How are multi-well glass bottom plates used?
Q 2: What are the advantages of using multi-well glass bottom plates compared to the standard glass bottom culture dishes?
   
Q 3: Can I get poly-d-lysine or collagen coated glass bottom multi-well plates?
Q 4: Who are the main users of multi-well glass bottom plates?
Q 5: What kind of feedback have you got from customers?
Q 6: What type of microscope is the best for multi-well glass bottom plates?
   
Q 7: How deep are the wells of the glass bottom multi-well plates? What is the height of the well sidewall?
   
Q 8: What is the diameter of the wells in the standard glass bottom multi-well plates?
   
Q 9: What is the chemical compatibility of the glass bottom dishes and multi-well plates? Can they be used with organic solvents?

Answers to Multi-Well FAQs
Q1 How are multi-well glass bottom plates typically used?
The Multi-Well Glass Bottom Dishes can be used for almost all of the same applications for which the standard glass bottom dishes can be used. The biggest applications are fluorescence/immunofluorescence microscopy, confocal, live cell imaging, microinjection, videomicroscopy, phase contrast and time lapse photomicroscopy.


Answers to Multi-Well FAQs

Q2 What are the advantages of using multi-well glass bottom plates compared to the standard glass bottom culture dishes?
A: The main advantage of the multi-well glass bottom plates is that you can grow 6, 12 ,24 or 96 cultures under identical conditions in the same culture plate. The multi-well plates are ideal for high throughput, high/high content screening applications.
B: Analysis using the multi-well plates is streamlined because only one (1) plate (versus multiple petri dishes) needs to be handled.
C: For a number of applications, treatment of the cultures (e.g., irradiation) is simplified using the multi-well plates.
D: Smaller wells in the glass bottom multi-well plates are useful for application of precious reagents in smaller volumes.


Answers to Multi-Well FAQs Q3 Can I get poly-d-lysine or collagen coated glass bottom multi-well plates?

Availability:
Currently, only poly-d-lysine coated 96-well plates are available as standard products (Part #'s: P96GC-0-5-F and P96GC-1.5-0-F).
Special Orders:
All uncoated plates are available coated on a special order basis. However, because no samples of coated multi-well plates are available, it is recommended that you request a sample of the uncoated multi-well plate (to inspect the geometry and/or apply your own coating) along with a coated glass bottom dish (to insure your cells will grow on the coated glass). This pre-testing is important because special order plates cannot be returned.
Pricing: Special order plates are $60/case above the corresponding uncoated multi-well plates. There is a $50 special order charge that is waived when 3 or more cases are ordered.
Delivery: Lead time for special orders is 6-8 weeks.


Answers to Multi-Well FAQs

Q4 Who are the main users of glass bottom multi-well plates?


Pharmaceutical Companies (due to high throughput testing), Academic/Research Institutions, and Medical Institutions.

Answers to Multi-Well FAQs

Q5 What kind of feedback have you receivedfrom initial customers?

Feedback has been very positive. A number of customers have purchased multi-well plates without first trying a sample based on their previous experience with the standard glass bottom dishes for new users.

For new users, a sample plate is available for evaluation.


Answers to Multi-Well FAQs

Q6 What type of microscope is the best for multi-well glass bottom plates?

The 6-well glass bottom plate can be used with upright or inverted microscopes. However, the 12-well, 24-well and 96-well plates are compatible with inverted microscopes.

Answers to Multi-Well FAQs

Q7: How deep are the micro-wells of the glass bottom multi-well plates? What is the height of the well sidewalls?

The depth of the micro-wells and the height of the sidewall depends on the type of multi-well plate:

1. 6-well plate: 1.45-1.55 mm/ 17 mm
2. 12-well plate: 1.45-1.55 mm/ 17 mm
3. 24-well plate: 1.10-1.20 mm/ 17 mm
4. 96-well plate: 1.05-1.25 mm/ 10 mm

Answers to Multi-Well FAQs

Q8 What is the diameter of the wells in the standard glass bottom multi-well plates?

1. 6-well plate: 10, 14, or 20 mm
2. 12-well plate: 10 or 14 mm
3. 24-well plate: 10 or 13 mm
4. 96-well plate: 5 mm

Answers to General FAQs
Q9: What is the chemical compatibility of the Glass Bottom Dishes and Multi-Well Plates? Can they be used with organic solvents?

A. The body of the glass bottom dishes and multi-well plates is made from polystyrene. Therefore, they have limited compatibility with organic solvents. Please see the chemical compatibility table.
Solvent Chemical Compatibility
Acetone Poor
Ammonium hydroxide (1N) Fair
Ammonium hydroxide (25%) Fair
Aniline Good
Butanol Good
Chloroform Poor
Dimethylformamide Poor
Dimethylsulfoxide(DMSO) Poor
DMSO/H2O (20/80) Good
Dioxane Poor
Ethanol Good
Hexane Poor
Hydrochloric acid (25%) Good
Hydrochloric acid (concentrated) Fair
Methanol Good
Methyl ethyl diketone Poor
Methylene chloride Poor
Nitric acid (25%) Poor
Nitric acid (concentrated) Poor
Sodium hydroxide Good
Toluene Poor
Xylene Poor

 

 
 
 

 

 

 

MatTek Corporation
200 Homer Avenue
Ashland, MA 01721
USA

Phone: 508.881.6771
Fax: 508.879.1532


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