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Click on a subject heading below to view questions and answers
relating to your selection. Links throughout the answers will guide you to further information
on our website or from other sources. Should you have any further questions, please contact
MatTek Corporation at DishInfo
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| Q |
1:How
are multi-well glass bottom plates used?
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| Q |
2:
What are the advantages of using multi-well glass bottom plates compared to the standard glass
bottom culture dishes?
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| Q |
3:
Can I get poly-d-lysine or collagen coated glass bottom multi-well plates?
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| Q |
4:
Who are the main users of multi-well glass bottom plates?
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5:
What kind of feedback have you got from customers?
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6:
What type of microscope is the best for multi-well glass bottom plates?
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| Q |
7:
How deep are the wells of the glass bottom multi-well plates? What is the height of the well
sidewall?
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| Q |
8:
What is the diameter of the wells in the standard glass bottom multi-well plates? |
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| Q |
9: What is
the chemical compatibility of the glass bottom dishes and multi-well plates? Can they be used
with organic solvents? |
Answers to Multi-Well FAQs |
Q1
How are multi-well glass bottom plates typically used?
The Multi-Well Glass Bottom Dishes can be used for almost all of the same
applications for which the standard glass bottom dishes can be used. The biggest applications
are fluorescence/immunofluorescence microscopy, confocal, live cell imaging, microinjection,
videomicroscopy, phase contrast and time lapse photomicroscopy.
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| Answers
to Multi-Well FAQs |
Q2 What are the advantages of using multi-well glass bottom plates compared to the standard
glass bottom culture dishes?
A: The main advantage of the multi-well glass bottom plates is that
you can grow 6, 12 ,24 or 96 cultures under identical conditions in the same culture plate.
The multi-well plates are ideal for high throughput, high/high content screening applications.
B: Analysis using the multi-well plates is streamlined because only one (1) plate (versus multiple
petri dishes) needs to be handled.
C: For a number of applications, treatment of the cultures (e.g., irradiation) is simplified
using the multi-well plates.
D: Smaller wells in the glass bottom multi-well plates are useful for application of precious
reagents in smaller volumes.
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| Answers
to Multi-Well FAQs |
Q3
Can I get poly-d-lysine or collagen coated glass bottom multi-well plates?
Availability:
Currently, only poly-d-lysine coated 96-well plates are available as standard products (Part
#'s: P96GC-0-5-F and P96GC-1.5-0-F).
Special Orders:
All uncoated plates are available coated on a special order basis. However, because no samples
of coated multi-well plates are available, it is recommended that you request a sample of the
uncoated multi-well plate (to inspect the geometry and/or apply your own coating) along with
a coated glass bottom dish (to insure your cells will grow on the coated glass). This pre-testing
is important because special order plates cannot be returned.
Pricing: Special order plates are $60/case above the corresponding uncoated multi-well
plates. There is a $50 special order charge that is waived when 3 or more cases are ordered.
Delivery: Lead time for special orders is 6-8 weeks.
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| Answers
to Multi-Well FAQs |
Q4 Who are the
main users of glass bottom multi-well plates?
Pharmaceutical Companies (due to high throughput testing),
Academic/Research Institutions, and Medical Institutions.
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| Answers
to Multi-Well FAQs |
Q5
What kind of feedback have you receivedfrom initial customers?
Feedback has been very positive. A number of customers have
purchased multi-well plates without first trying a sample based on their previous experience
with the standard glass bottom dishes for new users.
For new users, a sample plate
is available for evaluation.
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| Answers
to Multi-Well FAQs |
Q6
What type of microscope is the best for multi-well glass bottom plates?
The 6-well glass bottom
plate can be used with upright or inverted microscopes. However, the 12-well, 24-well and 96-well
plates are compatible with inverted microscopes.
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| Answers
to Multi-Well FAQs |
Q7:
How deep are the micro-wells of the glass bottom multi-well plates? What is the height of the
well sidewalls?
The
depth of the micro-wells and the height of the sidewall depends on the type of multi-well plate:
1. 6-well plate: 1.45-1.55 mm/ 17 mm
2. 12-well plate: 1.45-1.55 mm/ 17 mm
3. 24-well plate: 1.10-1.20 mm/ 17 mm
4. 96-well plate: 1.05-1.25 mm/ 10 mm
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| Answers
to Multi-Well FAQs |
Q8
What is the diameter of the wells in the standard glass bottom multi-well plates?
1. 6-well plate: 10, 14,
or 20 mm
2. 12-well plate: 10 or 14 mm
3. 24-well plate: 10 or 13 mm
4. 96-well plate: 5 mm
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| Answers
to General FAQs |
Q9: What is the chemical compatibility
of the Glass Bottom Dishes and Multi-Well Plates? Can they be used with organic solvents?
A. The body of the glass bottom
dishes and multi-well plates is made from polystyrene. Therefore, they have limited compatibility
with organic solvents. Please see the chemical compatibility table.
| Solvent |
Chemical Compatibility |
| Acetone |
Poor |
| Ammonium hydroxide (1N) |
Fair |
| Ammonium hydroxide (25%) |
Fair |
| Aniline |
Good |
| Butanol |
Good |
| Chloroform |
Poor |
| Dimethylformamide |
Poor |
| Dimethylsulfoxide(DMSO) |
Poor |
| DMSO/H2O
(20/80)
| Good |
| Dioxane |
Poor |
| Ethanol |
Good |
| Hexane |
Poor |
| Hydrochloric acid (25%) |
Good |
| Hydrochloric acid (concentrated) |
Fair |
| Methanol |
Good |
| Methyl ethyl diketone |
Poor |
| Methylene chloride |
Poor |
| Nitric acid (25%) |
Poor |
| Nitric acid (concentrated) |
Poor |
| Sodium hydroxide |
Good |
| Toluene |
Poor |
| Xylene |
Poor |
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