MICROSCOPIC TECHNIQUE TITLE HYPERLINKTR NO ABSTRACT KEYWORDS MATERIALS & METHODS JOURNAL VOL YEAR MONTH ISSUE MATTEK DISH  SPECIES TISSUE ATCC DESC MORPHOLOGY CELL LINE MAIN AUTHOR CONTRIBUTING AUTHORS COMPANY
                                       
Acoustic Microscopy                                       
  Optical And Ultrasonic Monitoring Of Laser-Generated Intracellular Contrast Agents: Initial Cell Culture Studies 384 Acoustically monitored laser-induced optical breakdown (LIOB) can be used its an important diagnostic and therapeutic tool in living cells. With properly controlled laser parameters, optical breakdown is a minimally invasive means to target a single, tran laser-induced optical
breakdown (LIOB), photodisruption		 CHO-K1 (Chinese hamster ovary) cells were used as the
in vitro target tissue for laser photodisruption. Cells were
cultured in a self-adherent monolayer onto a No. 0 coverslip
in a glass-bottom culture dish (MatTek Corporation,
Ashland, MA). Cells were gr		 Ultrasonics Symposium 2004 IEEE 2 2004 August     CHINESE HAMSTER OVARY ECACC 85051005
Morphology: Epithelial
Hamster Chinese ovary
Depositor: Dr D Newell, PHLS CAMR, Porton Down, Salisbury, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Nutritional and gene expression studies		 epithelial-like CHO Zohdy, Marwa J. Marwa J. Zohdy, Christine Tse, Jing Yong Ye, and Matthew O’Donnell University of Michigan
Analytical microscopy                                       
  Conformational Changes In Hiv-1 Gp41 In The Course Of Hiv-1 Envelope Glycoprotein-Mediated Fusion And Inactivation 364 HIV-1 envelope glycoprotein-mediated fusion is driven by the concerted coalescence of the HIV-1 gp41 N- and C-helical regions, which results in the formation of 6-helix bundles. These two regions are considered prime targets for peptides and antibodies th glycoprotein-mediated fusion, HIV-1-mediated cell-cell fusion, HIV-1 gp41, immunogenic HeLa cells were plated in 12 well plates, 105 cells per well, 24 h prior to
infection. The vaccinia stock (20 íL of approximately 109
plaque forming units/mL) was mixed with 30 íL of 0.25
mg/mL trypsin solution in Dulbecco’s phosphate-buffered
saline		           P35GCOL-0-10-C HUMAN cervix IZSBS BS TCL20
Morphology: epithelial-like
Species: human, Black female 31 years old; Tissue: cervix; Tumor: carcinoma, epitheloid
Properties: antitumour testing; transformation; tumorigenicity; cytotoxicity; cell biology; bacterial invasiveness; virology		 epithelial-like HeLa Dimitrov, Antony S. Antony S. Dimitrov, John M. Louis, Carole A. Bewley, G. Marius Clore, and Robert Blumenthal National Institutes of Health
  Mechanism Of Prion Propagation: Amyloid Growth Occurs By Monomer Addition Amyloid Growth Occurs By Monomer Addition 357 Abundant nonfibrillar oligomeric intermediates are a common feature of amyloid formation, and these oligomers, rather than the final fibers, have been suggested to be the toxic species in some amyloid diseases. Whether such oligomers are critical intermed prion inheritance, polymerization, NM, a fragment of the Sup35 protein containing
the glutamine/asparagine-rich N-terminal (N) and highly charged middle (M)
domains, amyloid formation		 For continuous thioflavin T assay measurements,  concentrated
stocks of NM stored in either 6 M GuHCl (fluorescently labeled NM
and NM used for Figures 2C, 2F, and 6) or 4 M GuHCl and 4 M urea
(NM used for all other experiments) were diluted at least 2		 PLoS Biology 2 2004 September 9 P35G-1.5-14-C         Coleoptiles victoriae from oat Collins, Sean R. Sean R. Collins, Adam Douglass, Ronald D. Vale, Jonathan S. Weissman HHMI
  Novel Dynamic Rheological Behavior Of Individual Focal Adhesions Measured Within Single Cells Using Electromagnetic Pulling Cytometry 426 The rheology of cells and sub-cellular structures, such as focal adhesions, are important for cell form and function. Here we
describe electromagnetic pulling cytometry (EPC), a technique to analyze cell rheology by applying dynamic tensional forces to
 Cell mechanics; Cytoskeleton; Focal adhesion; Creep; Magnetic Bovine capillary endothelial cells were cultured in
10% CO2 at 37  C on gelatin-coated tissue culture dishes
in Dulbeccos modified Eagles medium (DMEM,
Gibco-BRL) supplemented with 10% calf serum (CS;
Hyclone), 10 mM Hepes (JRH-Biosciences), 2 mM
L		 Acta Biomaterialia 1 2005 May 3   BOVINE       ALVEOLAR TYPE II Overby, Darryl R. Darryl R. Overby, Benjamin D. Matthews, Eben Alsberg, Donald E. Ingber Children's Hospital Boston
  Surface Probe Measurements Of The Elasticity Of Sectioned Tissue, Thin Gels And Polyelectrolyte Multilayer Films:Correlations Between Substrate Stiffness And Cell Adhesion 483 Surface probe measurements of the elasticity of thin film matrices as well as biological samples prove generally
important to understanding cell attachment across such systems. To illustrate this, sectioned arteries were probed by
atomic force microscop		 Surface structure, morphology, roughness, and topography; Adhesion; Atomic force microscopy A7r5 SMCs (aorta-derived cell line) were maintained
in polystyrene flasks between passages 2
and 15, and cultured in media containing DMEM,
supplemented with 10% of fetal bovine serum and
antibiotics. Cells were passed every 3 days, when
reaching 80		 Surface Science 570 2004 October 1-2     MUSCLE     A7R5 Engler, Adam J. Adam J. Engler, Ludovic Richert, Joyce Y. Wong,
Catherine Picart, Dennis E. Discher		 University of Pennsylvania
Axioscope Fluorescence Microscopy                                       
  Activation of erbB-1 Signaling in Tanycytes of the Median Eminence Stimulates Transforming Growth Factor 1 Release via Prostaglandin E2 Production and Induces Cell Plasticity 496 The activation of transforming growth factor  (TGF)– erbB-1 and neuregulin– erbB-4 signaling pathways in hypothalamic astrocytes
has been shown to play a key role in the process by which the neuroendocrine brain controls luteinizing hormone-releasing ho		 neuroendocrine; TGF ; TGF; ependymoglial cells; LHRH; cell plasticity; hypothalamus Tanycytes were seeded in cloning
cylinders positioned on 37 mm culture chambers (MatTek Corp., Ashland,
MA) containing poly-L-ornithine- and laminin-coated glass coverslips,
as described above. After removal of the cylinder, the cells were
maintained 		 Journal of Neuroscience 23 2003 November 33           Tanycytes Prevot, Vincent Vincent Prevot, Anda Cornea, Alison Mungenast, Gregory Smiley, and Sergio R. Ojeda Oregon Health and Sciences University
Calcium Imaging                                       
  Astragaloside IV inhibits spontaneous synaptic transmission and synchronized
Ca2+ oscillations on hippocampal neurons		 897 Aim: To investigate the changes in the spontaneous neuronal excitability induced
by astragaloside IV (AGS-IV) in the cultured hippocampal network. Methods:
Hippocampal neurons in culture for 9–11 d were used for this study. The spontaneous
synaptic activi		 astragaloside IV; synchronized spontaneous
Ca 2+ osci l l at ion; spont aneous synapt i c
transmission; voltage-gated Na+ currents;
voltage-gated K+ currents		 In brief, hippocampal
tissues from 18 d fetal rats...were then dissociated
by trituration with a glass Pasteur pipette and plated in 35
mm culture dishes with a glass bottom (MatTek, Ashland,
MA, USA) for culture and subsequent microscopy.		 Acta Pharmacologica Sinica 29 2008 January 1 35-mm glass bottom dishes         Hippocampal neurons from embryonic
Sprague-Dawley rats (E18)		 Shao-qing ZHU Shao-qing ZHU, Lei QI, Yan-fang RUI, Ru-xin LI, Xiang-ping HE, Zuo-ping XIE  
  Hydroxylation Increases the Neurotoxic Potential of BDE-47 to Affect
Exocytosis and Calcium Homeostasis in PC12 Cells		 881 BACKGROUND: Oxidative metabolism, resulting in the formation of hydroxylated polybrominated
diphenyl ether (PBDE) metabolites, may enhance the neurotoxic potential of brominated flame
retardants.
OBJECTIVE: Our objective was to investigate the effects of 		 Bioactivation, brominated flame retardants, calcium, catecholamine, exocytosis, intracellular
calcium stores, neurotoxicity, neurotransmitter release, persistent organic pollutants, polybrominated
diphenyl ether.		 For Ca2+ imaging
experiments, we subcultured undifferentiated
PC12 cells in poly-L-lysine–coated
glass-bottom dishes (MatTek, Ashland, MA,		 Environmental Health Perspectives 116 2008 May 5 poly-L-lysine–coated
glass bottom dishes		         PC12 Milou M.L. Dingemans Milou M.L. Dingemans, Aart de Groot, Regina G.D.M. van Kleef,  Åke Bergman, Martin van den Berg,
Henk P.M. Vijverberg, and Remco H.S. Westerink		  
  Neonatal Exposure to Brominated Flame Retardant BDE-47 Reduces Long-Term
Potentiation and Postsynaptic Protein Levels in Mouse Hippocampus		 882 BACKGROUND: Increasing environmental levels of brominated flame retardants raise concern about
possible adverse effects, particularly through early developmental exposure.
OBJECTIVE: The objective of this research was to investigate neurodevelopmental mec		 αCaMKII, brain growth spurt, developmental neurotoxicity, field-EPSP recording,
hippocampal synaptic plasticity, postsynaptic density.		 PC12 cells were subcultured in
poly-L-lysine–coated glass-bottom cell culture
dishes (MatTek, Ashland MA, USA)		 Environmental Health Perspectives 115 2007 June 6 poly-L-lysine–coated glass bottom
dishes		         PC12 Milou M.L. Dingemans Milou M.L. Dingemans, Geert M.J. Ramakers, Fabrizio Gardoni, Regina G.D.M. van Kleef, Åke Bergman,
Monica Di Luca, Martin van den Berg, Remco H.S. Westerink, and Henk P.M. Vijverberg		  
  Pregnancy-Specific Changes in Uterine Artery
Endothelial Cell Signaling in Vivo Are Both Programmed
and Retained in Primary Culture		 887 Ovine uterine artery (UA) endothelial cells (UAEC) maintained
in culture to passage 4 retain pregnancy-specific
changes in vasodilator production, which in turn is associated
with differences in Ca2 and ERK 1/2 signaling. The question
remains whether thi		 Ovine uterine artery (UA) endothelial cells (UAEC), Pregnancy-specific changes Glass-bottom microwell dishes
for Ca2 imaging studies were purchased from MatTek Corporation
(Ashland, MA),		 Journal of Endocrinology 144 2003   8 glass bottom dishes         UAEC Shannon M. Gifford Shannon M. Gifford, Jackie M. Cale, Stephen Tsoi, Ronald R. Magness, and Ian M. Bird  
Confocal Fluorescence                                       
  Molecular Perspective of Antigen-mediated Mast Cell Signaling 907 Agonist activation regulates reciprocal interactions of spinophilin
and arrestin with the 2A- and 2B-adrenergic receptor (AR)
subtypes via their 3i loop. Because arrestin association with G
protein-coupled receptor is preceded by redistribution of arres		   HEK 293 or MEFs were plated the night
before the assay on MatTek dishes (35 mm) coated with 2.5 g/cm2
poly-D-lysine		 Molecular Pharmacology 67 2005 May 5 35-mm glass bottom dishes         HEK 293 or MEFs Ashley E. Brady Ashley E. Brady, Qin Wang, Patrick B. Allen, Mark Rizzo, Paul Greengard, and
Lee E. Limbird		  
Confocal Laser                                       
  Close Homolog of L1 is an Enhancer of Integrin-mediated Cell Migration 879 Cells actively produce contractile forces for a variety of processes including cytokinesis and motility. Contractility is
known to rely on myosin II motors which convert chemical energy from ATP hydrolysis into forces on actin filaments. However, the
basi		 Actin, Active cytoskeletal networks, Contractility, Myosin II, Xenopus, alpha-actinin Deposited onto an inert oil layer (Fluorinert FC-40,
3M, St. Paul, MN; cat. No. F9755, Sigma, St. Louis, MO) on the bottom of a
MatTek dish (model No. P35G-1.5-14-C, MatTek, Ashland, MA) equipped
with a lid to minimize evaporation (Fig. 1 A).		 Biophysical Journal 94 2008 April 8 P35G-1.5-14-C         M-phase extracts (Xenopus) Poul M. Bendix Poul M. Bendix, Gijsje H. Koenderink, Damien Cuvelier, Zvonimir Dogic, Bernard N. Koeleman,
William M. Brieher, Christine M. Field, L. Mahadevan, and David A. Weitz		  
Confocal Laser Scanning                                       
  Phosphorylation-dependent Localization
of Microtubule-associated Protein
MAP2c to the Actin Cytoskeleton		 750 IB kinase 2 (IKK2 or IKK) is a component of the IKK complex that coordinates the cellular response to a diverse set of extracellular
stimuli, including cytokines, microbial infection, and stress. In
response to an external stimulus, the complex is activa		 Aurora A  mitosis  NF-B  spindle polarity HeLa cells expressing GFP-a-tubulin where reverse-transfected with siRNA IKK2 no. 2. Briefly, 80 nmol of the siRNA was diluted in 500 ml of OptiMem (Invitrogen) and placed in a 35-mm glass bottom dish (MatTek). PNAS 104 2007 October 43 35-mm glass bottom dishes Human Cervix   Epithelial HeLa Irelan, Jeffrey T. Jeffrey T. Irelan*, Thomas J. Murphy*, Paul D. DeJesus*, Hsiangling Teo, DingYue Xu, Maria A. Gomez-Ferreria‡,
Yingyao Zhou*, Loren J. Miraglia*, Daniel R. Rines*, Inder M. Verma§, David J. Sharp‡, Vinay Tergaonkar§,
and Sumit K. Chanda		 Genomics Institute of the Novartis Research Foundation, †Institute for Molecular and Cell Biology, Albert Einstein College of Medicine, §The Salk Institute for Biological Studies
Confocal Microscopy                                       
  2-Adrenergic Agonist Enrichment of Spinophilin at the Cell
Surface Involves Subunits of Gi Proteins and Is
Preferentially Induced by the 2A-Subtype		 787 The HIV-1 Rev protein is an essential regulator of the HIV-1 mRNA expression that promotes the export of unspliced and partially
spliced mRNA. The export receptor for the leucine-rich nuclear
export signal (NES) of Rev has recently been recognized as CRM1		   HLtat cells were seeded onto coated
50-mm glass-bottom microwell dishes (MatTek), cultured in
phenol red-free DMEM [supplemented with 10% (volvol)
FCS]		           50-mm glass-bottom microwell dishes         Hltat Daelemans, Dirk Dirk Daelemans*, Elena Afonina‡, Jakob Nilsson§, Gudrun Werner, Jørgen Kjems§, Erik De Clercq*,
George N. Pavlakis‡, and Anne-Mieke Vandamme		 Human Retrovirus Section, National Cancer
Institute, University of Aarhus, Novartis Research Institute
  A Quantitative Analysis of Contractility in Active Cytoskeletal
Protein Networks		 759 The human EGF receptor (HER) 2 receptor tyrosine kinase is a survival factor for human cardiomyocytes, and its inhibition may
explain the increased incidence of cardiomyopathy associated with
the anti-HER2 monoclonal antibody trastuzumab (Genentech,
South		 monoclonal antibodies Au565, MCF7, MCF7/HER2, and HMCs cells
were seeded on glass bottom dishes (MatTek, Ashland, MA) the
day before the experiment.		 PNAS 104 2007 June 25 P35GC-1.0-14-C, P35G-1.0-14-C Human Breast cancer, Cardiomyocytes     Au565, MCF7, MCF7/HER2, and HMCs Spector, Neil L. Neil L. Spector*, Yosef Yarden, Bradley Smith‡, Ljuba Lyass§, Patricia Trusk§, Karen Pry§, Jason E. Hill§, Wenle Xia*,
Rony Seger, and Sarah S. Bacus		 Duke University Medical Center, Weizmann Institute, Cell Signaling
Technologies, §Targeted Molecular Diagnostics
  A role for IB kinase 2 in bipolar spindle assembly 747 Visual and auditory hallucinations accompany certain neuropsychiatric disorders, such as schizophrenia, and they also can be
induced by the use or abuse of certain drugs. The heptahelical
serotonin 2A receptors (5-HT2ARs) are molecular targets for drugind		 5-HT2A receptor  G protein-coupled receptor  internalization
MAP kinase  schizophrenia		 Neurons were grown on poly-L-lysine-coated glass coverslip culture plates (Mat-
Tek) at 37°C and in 5% CO2.		 PNAS 105 2008 January 3 Glass coverslip culture plates Mouse cortical neuronal     MEF Schmid, Cullen L. Cullen L. Schmid, Kirsten M. Raehal, and Laura M. Bohn Ohio State University College of Medicine
  A synthetic HIV-1 Rev inhibitor interfering with the CRM1-mediated nuclear export 771 Vaccination against amyloid -peptide (A) has been shown to be successful in reducing A burden and neurotoxicity in mouse
models of Alzheimer’s disease (AD). However, although A immunization
did not show T cell infiltrates in the brain of these mice,
an A 		 amyloid -peptide  encephalitis  vaccination Microglia were cultured on MatTek Petri dishes for 24 h in the presence and absence of IFN-g. PNAS 103 2006 March 13   Mouse Microglial       Monsonego Alon Monsonego*, Jaime Imitola‡, Sanja Petrovic‡, Victor Zota‡, Anna Nemirovsky*, Rona Baron*, Yair Fisher*,
Trevor Owens§, and Howard L. Weiner		 Ben-Gurion University, Brigham and Women’s Hospital, Harvard Medical School, University of Southern Denmark
  Activation of AMP-activated protein kinase by human EGF receptor 2/EGF receptor tyrosine kinase
inhibitor protects cardiac cells		 746 Vascular dysfunction has been reported in human cases of anthrax, in mammalian models of Bacillus anthracis, and in animals injected
with anthrax toxin proteins. To examine anthrax lethal toxin
effects on intact blood vessels, we developed a zebrafish mod		 endothelial  vascular permeability  VEGF For confocal imaging of the heart: 15 minutes
after bead injection, embryos were fixed in 2% PFA for 5–10 min; then
embedded in 2% low melt agarose (Bio-Rad) on 35-mm glass bottom microwell
dishes (MatTek Corp.).		 PNAS 105 2008 February 7 35-mm glass bottom microwell
dishes		 Zebrafish Heart   Endothelial       Harvard Medical School
  Agonist-directed signaling of the serotonin 2A receptor depends on -arrestin-2 interactions in vivo 845 Altered calcium homeostasis is implicated in the pathogenesis of Alzheimer’s disease (AD). Much effort
has been put into understanding the association between protein mutations causative of this devastating
neurodegenerative disease and perturbed calcium 		 Alzheimer’s disease, APPswe, Ca, Spontaneous calcium oscillation, Hippocampal neuron Mechanically dispersed cells were plated on
poly-d-lysine-coated 3.5cm glass bottom Petri dishes (Mattek Corporation)
at a density of 450,000 cells/dish and cultured for 12–14
days at 37 ◦C and 5% CO2.		 Journal of Neuroscience 436 2008 May 2 P35G-0-14-C         Ppswe Ewa Kloskowska Ewa Kloskowska,, Katarzyna Malkiewicza, Bengt Winblada,
Eirikur Benedikza, Joseph D. Bruton		  
  A-induced meningoencephalitis is IFN-dependent and is associated with T cell-dependent clearance
of A in a mouse model of Alzheimer’s disease		 877 Assessment
of fat-specific protein 27 in the adipocyte lineage suggests a dual
role for FSP27 in adipocyte metabolism and cell death. Am J Physiol
Endocrinol Metab 294: E654–E667, 2008. First published January 15,
2008; doi:10.1152/ajpendo.00104.2007.—Fat		 insulin; tumor necrosis factor-; adipogenesis; cell death-inducing
DFF45-like effector; apoptosis; 3T3-L1		 Confocal imaging studies using MitoTracker
Red CMX Ros (Invitrogen) were conducted on live cells by plating
3T3-L1 preadipocytes or COS cells onto laminin-coated MatTek glass
bottom dishes (MatTek, Ashland, MA)		 American Journal of Physiology: Endocrinology and Metabolism 294 2008 April 4 glass bottom dishes         3T3-L1 preadipocytes or COS cells Ji Young Kim Ji Young Kim, Kun Liu, Shengli Zhou, Kristin Tillison, Yu Wu, and Cynthia M. Smas  
  Anthrax lethal toxin induces cell death-independent permeability in zebrafish vasculature 816 Caveolin-1 is a principal component of caveolae membranes that may function as a transformation suppressor. For example, the human caveolin-1 gene is localized
to a suspected tumor suppressor locus (D7S522; 7q31.1)
that is deleted in human cancers, includ		   MTLn3 cells
were plated on collagen I-coated MATTEK tissue culture dishes at a
density of 2,500 cells/cm2.		 Journal of Biological Chemistry 275 2000   27   Mouse Caveolin     MTLn3 Zhang, Wei Wei Zhang, Babak Razania, Yoram Altschuler, Boumediene Bouzahzah,
Keith E. Mostov, Richard G. Pestell, and Michael P. Lisanti		 Albert Einstein College of Medicine, University of California, San Francisco
  APPswe mutation increases the frequency of spontaneous
Ca2+-oscillations in rat hippocampal neurons		 711 Background: The interaction between macrophages and Cryptococcus neoformans (Cn) is critical
for containing dissemination of this pathogenic yeast. However, Cn can either lyse macrophages or
escape from within them through a process known as phagosomal ex		 vacuole formation, Cryptococcus neoformans, murine macrophages Cells were then plated on polylysine
coverslip-bottom MatTek plates (MatTek Cultureware,
Ashland MA) at a density of 5 × 104 per plate in feeding
media,		 BioMedCentral Immunology 8 2007 August 16 P35GC-0-14-C Mice macrophage-like     J774.16 Alvarez, Mauricio Mauricio Alvarez, Arturo Casadevall Albert Einstein College of Medicine
  Assessment of fat-specific protein 27 in the adipocyte lineage suggests a dual
role for FSP27 in adipocyte metabolism and cell death		 853 Lipoteichoic acid (LTA) is a central inducer
of inflammatory responses caused by Grampositive
bacteria, such as Staphylococcus aureus,
via activation of TLR2. Localization of TLR2 in
relation to its coreceptors may be important for
function. This study ex		 TLR, LTA, endocytosis MDCK cells stably expressing Eea-1GFP were transiently transfected with the
TLR2 overnight in Microwell dishes for microscopy (MatTek Corp.)		 Journal of Leukocyte Biology 84 2008 July   35-mm glass bottom dishes         HEK293, MDCK Nadra J. Nilsen Nadra J. Nilsen,* Susanne Deininger,† Unni Nonstad,* Frode Skjeldal,‡ Harald Husebye,*
Dmitrii Rodionov,‡ Sonja von Aulock,† Thomas Hartung,†,§ Egil Lien,¶ Oddmund Bakke,‡
and Terje Espevik		  
  Caveolin-1 Inhibits Epidermal Growth Factor-stimulated Lamellipod Extension and Cell Migration in Metastatic
Mammary Adenocarcinoma Cells (MTLn3)		 827 The molecular mechanisms by which the AP-1 transcription factor c-Jun exerts its biological functions are not clearly
understood. In addition to its well established role in transcriptional
regulation of gene expression, several reports
have suggested tha		   For photoswitch and fluorescence recovery after photobleaching
(FRAP) studies, cells were transfected with PS-CFP2-
DDX21, EGFP-DDX21 (15), or EGFP-DDX21 mut and grown
on glass-bottom dishes (MatTek).		 Journal of Biological Chemistry 283 2008 March 11 P50G-1.5-14-F Mouse Embryo     HeLa, MEF Holmstro, Tim H. Tim H. Holmstro¨m‡§, Antoine Mialon‡, Marko Kallio‡§, Yvonne Nymalm, Leni Mannermaa‡, Tina Holm,
Henrik Johansson, Elizabeth Black**, David Gillespie**‡‡, Tiina A. Salminen, U¨ lo Langel, Benigno C. Valdez§§,
and Jukka Westermarck		 University of Turku and Åbo Akademi University, University of Tampere and the Tampere University Hospital, State Technical
Research Center of Finland, University of Stockholm, University of Glasgow, University of Texas,MDAnderson Cancer Center
  Cell-to-cell spread and massive vacuole formation after Cryptococcus neoformans infection of murine macrophages It is increasingly recognized that the compartmental organization of signaling processes has a profound influence on cellular behavior. However, our inability to influence these compartmental events in a spatially restricted and acute manner limits our un   Transfected HeLa cells were grown on MatTeK dishes.           MatTeK dishes Human       HeLa Fili, N. N. Fili, V. Calleja, R. Woscholski, P. J. Parker,, and B. Larijani Cancer Research UK, Imperial College London
  Cellular trafficking of lipoteichoic acid and Toll-like receptor 2
in relation to signaling; role of CD14 and CD36		 795 Purpose: Clinical confocal scanning laser microscopes are used in vivo to examine the cornea. However, such instruments are unable to yield the precision and resolution provided by research level in vitro instruments. This study was carried out to evaluat cornea: basic science • cornea: epithelium • mitochondria Following the labeling with fluorescent dyes, the corneal buttons were rinsed twice in HBSS and mounted in 1% agarose, previously dissolved in HBSS, on glass–bottom plates (MatTek Corp., Ashland, MA). Investigative Opthamology & Visual Science   2006     P06G-1.5-20-F Bovine Cornea   Epithelial   Bantseev, V. V. Bantseev and J.G. Sivak University of Waterloo
  c-Jun Supports Ribosomal RNA Processing and Nucleolar Localization of RNA Helicase DDX21 724 In principle, cell surface receptors that are
overexpressed in tumor tissue could serve as targets for
anticancer drugs attached to receptor ligands. The purpose of
this paper is to identify the necessary elements for a successful
receptor-targeted drug. 		   Cells were grown in
coated 50-mm cover glass bottom dishes (MatTek, Ashland,
MA) in medium without phenol red.		 PNAS 95 1998 September 20 50-mm cover glass bottom dishes Human gastrin I     NIH/3T3 Czerwinski, Grzegorz GRZEGORZ CZERWINSKI, NADYA I. TARASOVA, AND CHRISTOPHER J. MICHEJDA* National Cancer Institute, Frederick Cancer Research and Development Center
  Compartmental signal modulation: Endosomal phosphatidylinositol 3-phosphate controls endosome morphology and selective cargo sorting 830 Acoustic overstimulation and ototoxic drugs can cause permanent hearing loss as a result of the damage and death of cochlear hair cells.
Relatively little is known about the signaling pathways triggered by such trauma, although a significant role has been		 cochlea; ototoxicity; calcium signaling; purinergic receptors; cell– cell communication; ERK The cochlear turns were placed onto Cell-Tak cell and tissue
adhesive (BD Biosciences, San Jose, CA)-coated MatTek (Ashland, MA)
dishes.		 Journal of Neuroscience 19 2008 May   P50G-0-14-F         Cochlea Manuela Lahne Manuela Lahne and Jonathan E. Gale  
  Confocal Laser Scanning Microscopy Imaging of the Bovine Corneal Epithelium in vitro 743 Va14Ja18 natural T (NKT) cells play an immunoregulatory role, which is controlled by a self glycolipid(s) presented by CD1d.
Although the synthetic antigen -D-galactosylceramide (-D-Gal-
Cer) stimulates all Va14Ja18 NKT cells, -anomeric D-glycosylceram		   Cells (5  106) were
plated in glass-bottom microwells (MatTek) and incubated
overnight at 37°C to allow the cells to adhere to the wells.		 PNAS 100 2003 February 4 glass bottom dishes Mouse       Va14Ja18 NKT Stanic, Aleksandar Aleksandar K. Stanic*, A. Dharshan De Silva*, Jang-June Park*, Venkataraman Sriram‡§, Shinichi Ichikawa,
Yoshio Hirabyashi, Kyoko Hayakawa, Luc Van Kaer*, Randy R. Brutkiewicz‡§, and Sebastian Joyce		 Vanderbilt University School of Medicine, Yeshiva University, Indiana University School of Medicine, Walther Cancer Institute, Brain Science Institute, Fox Chase Cancer Center
  Cytotoxic agents directed to peptide hormone receptors: Defining the requirements for a successful drug 874 Calcium influx through long-lasting (“L-type”) Ca2 channels
(CaV) drives excitation-contraction in the normal heart. Dysregulation
of this process contributes to Ca2 overload, and
interventions that reduce expression of the pore-forming 1
subunit may a		 Functional Ca2+ Channels, Mutant B2, Cardiac Cells After trypsinization,
dissociated cells were plated in glass culture dishes (MatTek, Ashland, MA) for electrophysiology
(60–80  103 cells/ml).		 Journal of Pharmacology and Experimental Therapeutics 325 2008 April 1 glass bottom dishes         HL-1 cells Sabine Telemaque Sabine Telemaque, Swapnil Sonkusare, Terrie Grain, Sung W. Rhee, Joseph R. Stimers,
Nancy J. Rusch, and James D. Marsh		  
  Damage-Induced Activation of ERK1/2 in Cochlear Supporting Cells Is a Hair Cell Death-Promoting Signal That Depends on Extracellular ATP and Calcium 903 The spatial distributions of zinc, a representative transition metal, and active biomass in bacterial biofilms
were determined using two-photon laser scanning microscopy (2P-LSM). Application of 2P-LSM permits
analysis of thicker biofilms than are amenabl		   Thin biofilms were grown in microwell dishes with 0.17-mm glass coverslips at the bottom (MatTek, Ashland,
MA).		 Applied and Environmental Microbiology 71 2005 July 7 glass bottom dishes, 1.7mm thickness         PHL628 Zhiqiang Hu Zhiqiang Hu, Gabriela Hidalgo, Paul L. Houston, Anthony G. Hay, Michael L. Shuler,
He´ctor D. Abrun˜a, William C. Ghiorse, and Leonard W. Lion		  
  Defective presentation of the CD1d1-restricted natural Va14Ja18 NKT lymphocyte antigen caused
by -D-glucosylceramide synthase deficiency		 837 A mast cell-based biosensor has been developed to enable the use of these cells in numerous applications including pharmaceutical screening,
environmental monitoring, clinical diagnosis and homeland security. Rat basophilic leukemia (RBL) mast cells offer		 IgE receptors; Immunosensor; Bacterial diagnostics; Calcium responses; CD14 chimeric protein For confocal
fluorescence experiments, 2.0mL of this cell suspension was
plated into glass bottom Petri dishes (MatTek Corp.		 Biosensors and Bioelectronics 23 2008 February 7 P35G-1.5-14-F         RBL-2H3 Theresa Curtis Theresa Curtis a,∗, Rose Mary Z.G. Naal b,1, Carl Batt c, Joel Tabb a, David Holowka  
  Design of Mutant 2 Subunits as Decoy Molecules to Reduce
the Expression of Functional Ca2 Channels in Cardiac Cells		 871 To apply siRNA as a therapeutic agent, appropriate attention should be paid to the optimization of the siRNA gene silencing effect, both in terms of magnitude and duration. Intracellular time-controlled siRNA delivery could aid in tailoring the kinetics o Biodegradable materials • Controlled release • Microgels • siRNA • Drug delivery • Biomedical applications HUH7 cells were seeded in sterile glass-bottomed
culture dishes (MatTek Corporation, Ashland, MA) at a density of
3104 cells per cm2 and allowed to attach overnight.		 Advanced Functional Materials 18 2008 April 7 glass bottom dishes         HUH7 Koen Raemdonck Koen Raemdonck , Tinneke G. Van Thienen , Roosmarijn E. Vandenbroucke , Niek N. Sanders, Joseph Demeester , Stefaan C. De Smedt  
  Determination of Spatial Distributions of Zinc and Active Biomass in
Microbial Biofilms by Two-Photon Laser Scanning Microscopy		 712 Stargazin is an accessory protein of AMPA receptors that
enhances surface expression and also affects the biophysical
properties of the receptor. AMPA receptor domains necessary
for either of these two processes have not yet been identified.
Here, we used		   Collagen- or poly-Dlysine-
coated 14-mm glass bottom culture dishes (MatTek
Corp., Ashland, MA) were incubated with ECL Attachment
Matrix (Upstate Cell Signaling Solutions, Lake Placid, NY) for
1 h at 37 °C then washed with cMEM before plating cells.		 Journal of Biological Chemistry 281 2006 August 33 P35GC-0-14-C Human Embryonic Kidney Fibroblasts     HEK-293 Bedoukian, Matthew A. Matthew A. Bedoukian, Autumn M. Weeks, and Kathryn M. Partin Colorado State University
  Development of a mast cell-based biosensor 825 Lysophosphatidic acid (LPA) stimulates cells by activation of five Gprotein coupled receptors, termed LPA1-5. The LPA1 receptor is the most widely expressed and is a major regulator of cell
migration. In this study, we show that
phorbol ester (PMA)-induce		   HeLa cells were
plated onto 35mm glass bottom microwell
dishes (MatTek Corp., Ashland, MA).		 Journal of Biological Chemistry 283 2008 February 9           HeLa Urs, Nikhil M. Nikhil M. Urs, Andrew P. Kowalczyk and Harish Radhakrishna Georgia Institute of Technology, Emory University
  Dextran Microgels for Time-Controlled Delivery of siRNA 793 Purpose:The purpose of this study was to investigate the feasibility of using confocal laser scanning microscopy together with specific fluorescent markers to assess the distribution of cellular organelles in corneal epithelium of rat in vivo.

Methods:F		 cornea: basic science • mitochondria • imaging/image analysis: non-clinical Animals (n=5) were then placed on a custom-designed heated microscope stage (to maintain body temperature) with the cornea facing glass-bottom multi-well plates (MatTek Corp., Ashland, MA).           P06G-1.5-20-F Rat Cornea   Epithelial   Bantseev, V. V. Bantseev, D. McCanna, J.-Y. Driot, K. W. Ward and J. G. Sivak University of Waterloo, Bausch&Lomb, Inc.
  Different Domains of the AMPA Receptor Direct Stargazin-mediated Trafficking and Stargazin-mediated Modulation of Kinetics 841 Many cell types, including neurons and epithelial cells, express a variety of annexins.
Although the overall function has only been partially unravelled, a dominant feature is the
formation of two-dimensional assemblies under the plasma membrane in a calc		 Annexin, Calcium binding, FRAP, FRET, Translocation For imaging experiments, cells were plated in
35 mm MatTek chambers (Ashland, MA) and transfected with
FuGENE 6 reagent (Roche, Mannheim, Germany) at 50%
confluency.		 Experimental Cell Research 314 2008 March 5 35-mm glass bottom dishes         N1E-115 Tatsiana Skrahina Tatsiana Skrahina1, Alen Piljić1, Carsten Schultz