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How do you know
which Glass Bottom Culture Dish to choose?
This fast question/answer session will help you select the proper dish for your application.
1. Should I use 35 mm or 50 mm diameter dishes, or one of the
multi-well plates?
The 35 mm dishes are used for most applications. The 50 mm dishes are usually chosen for
microinjection work because of their larger diameter and
lower side walls that allow for easier access to the cells.
Sample Part Numbers
P35G-0-10-C, P50G1.5-14-F
For customers who want to prepare a large number of identical cultures, you may want to consider
our 6-, 12-, 24-, or 96-well glass bottom plates.
Sample part #s: P06G-0-10-F, P12G-0-14-F.
2. What coverslip thickness should I use?
Glass bottom dishes are available with No. 0, No. 1.0, and No. 1.5 coverslips. In many instances,
your microscope manufacturer will specify coverslip
thickness (No.) for your microscope model and the specific objective you're using. The No. 0
coverslip gives you the most working distance.
Some objectives are designed to work best with a No. 1.0 coverslip.
Sample part #s: P35G-0-10-C, P50G-1.5-14-F.
3. What diameter microwell should I use, 10 mm, 14 mm, or 20 mm?
For most applications, a 14 mm glass microwell is sufficient. Choose the 20 mm diameter microwell
to maximize viewing area. If your application is
cell-limited and/or you are concerned about media or reagent consumption, then you should choose
the 10 mm diameter microwell.
Sample part #s: P35G-0-14-C, P35G-1.5-20-C.
4. Should I use a coated or uncoated coverslip? If I need to use a coated coverslip, should
I use the poly-d-lysine coating or the collagen coating?
If your cells grow well on uncoated glass, then order the dishes that come with an uncoated
coverslip (P35G and P50G series). If your cells do not grow on
uncoated glass, try our poly-d-lysine coated (P35GC and P50GC series) or collagen
coated dishes (P35GCOL series).
Poly-d-lysine is used for neuronal research since most neurons will not grow on un-coated
glass. It is also useful for other primary cells as well. For other
cell types such as endothelial, hepatocytes or muscle cells, collagen coating helps with cell
attachment, spreading and rapid expansion of cell populations.
All of our coated dishes are lot certified with primary hippocampal neurons.
Sample part #s: P35GC-0-14-C, P35GCOL-1.5-10-C
5. Should I use a dish with a gridded coverslip?
Some researchers would like to microinject specific cells and then be able to monitor individual
cells as a function of time in culture. The use of gridded
glass bottom coverslip dishes greatly facilitates such studies. See part #: P35G-2-14-C-GRID
or P50G-2-14-F-GRID. Both products contain
photo-etched gridded coverslips (No. 2 thickness, alphanumeric grid of 520 coded squares. Each
square is 600 x 600 um) from Bellco Glass.
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